Best Info About How To Check Primer Dimer
Enter one or both primer sequences in the primer parameters section of the form.
How to check primer dimer. Enter the sequence of your forward primer into the sequence box, and then click. Click the 'get primers' button to submit the search and retrieve template and specificity information. For analyzing and comparing multiple primer sequences simultaneously.
Detection [ edit] primer dimers may be visible after gel electrophoresis of the pcr product. The analyzer accepts text and table. One or more primer sequences go to the primer blast submission form.
As ehsan said it's a good way to see primer dimers. A target template sequence or accession number. If only one primer is available,.
Write or paste your primer sequences to the input field (upper window). Primer dimers will still form in the negative control.